Ultraviolet–visible spectroscopy or ultraviolet–visible spectrophotometry (UV–Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible spectral regions. This means it uses light in the visible and adjacent ranges. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, atoms and molecules undergo electronic transitions. Absorption spectroscopy is complementary to fluorescence spectroscopy, in that fluorescence deals with transitions from the excited state to the ground state, while absorption measures transitions from the ground state to the excited state.
Applications
An example of a UV/Vis readout
UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.
UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.
| BDI-E01 Microprocessor UV/VIS Spectrophotometer |
| (Single Beam) range 190 to 1000nm with facility for automatic concentration, % Transmission, Absorbance, and K factor measurements. It has a 16 x 2 line LCD data display and motorized wavelength selection on 4 digits LED display. It has quad sample holder with 4 position selector control for 10mm path length cuvettes. The facility has been provided for 100 sample data storage and interfaces with any Centronics printer. RS 232C interface can be provided in the request, A set of 2 quartz cuvettes is provided. |
| BDI-E02 Microprocessor UV/VIS Spectrophotometer |
| (Single beam) with range 340-960nm and facility for auto concentration, %Transmission, Absorbance, and K-factor measurements. It has a 16 x 2 line LCD for data & wavelength display. Its sample holder accommodates glass cuvettes as well as glass tubes of 10mm path length. The facility has been provided for 100 sample data storage and interfaces for any Centronics printer. RS 232C interface can be provided on request. A set of 2 glass cuvettes of 10mm path length is provided. |
| BDI-E03 Digital UV-VIS Spectrophotometer (Single Beam) |
| Range 190 to 1000nm with facility for automatic concentration, % Transmission & Absorbance measurements. Motorized microcontroller-based wavelength selection, 4 digits LED display for wavelength, 3½ digits LED data display. It has 4 position sample holder with position selector control for 10mm path length cuvettes. |
